Vitrification is a procedure that is utilized in the embryo and egg solidifying process so they can be put away for later use. It is a technology that has numerous utilizations outside of fertility care with egg and embryo solidifying, as it enables something with a crystalline structure to be changed over into something extremely smooth.

Vitrification utilizes streak cooling the oocytes by directly inundating it into fluid nitrogen. The ideal temperature to be accomplished is – 196ºC as at this temperature all the organic action stops. By inundating the cell directly into fluid nitrogen, this temperature is immediately accomplished when contrasted with slow freezing which may take substantially more time.

The procedure of vitrification is as per the following:

1. Ovarian Stimulation: It is the main step for the vitrification procedure. Hormones are managed to create numerous eggs one after another as compared to a one egg.
2. Egg assortment: When the follicles are prepared, the eggs are gathered. This generally occurs following 10 to 14 days of ovary stimulation and is done under sedation. One of the most widely recognized strategies for egg assortment is transvaginal ultrasound in which ultrasound is utilized to distinguish follicles and afterward a needle is utilized to recover the eggs.
3. The eggs are then dunked into cryoprotectants, which expel the water from the cell to avoid ice development during freezing. Ice-generation may even prompt cell break and consequently should be averted for effective freezing.
4. Eggs are then cooled quickly and put away in fluid nitrogen at a temperature of – 196ºC.

The eggs can be kept frozen from the above method for a long time. At the point when pregnancy is wanted, the eggs are defrosted and utilized as required.